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osteoblast differentiation medium  (Lonza)


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    Lonza osteoblast differentiation medium
    Osteoblast Differentiation Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+differentiation+medium/osteoblast+differentiation+medium/pm40362457-488-8-11
    Average 90 stars, based on 1 article reviews
    osteoblast differentiation medium - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Garlic extract enhances bioceramic bone scaffolds through upregulating ALP & BGLAP expression in hMSC-monocyte co-culture.
    Article Snippet: Bone homeostasis is predicated by osteoblast and osteoclast cell cycles where gene expressions are responsible for their differentiation from human mesenchymal stem cells (hMSC) and monocytes, respectively.. The proosteogenic potential of an hMSC-monocyte co-culture can be measured through complementary DNA (mRNA synthesis) within the nucleus, known as quantitative polymerase chain reaction (qPCR).. Through this technique, the effects of garlic extract (allicin) release from calcium phosphate bone scaffolds on gene expression of bone forming and bone remodeling cells was explored.

    Article Title: Biodentine Stimulates Calcium-Dependent Osteogenic Differentiation of Mesenchymal Stromal Cells from Periapical Lesions.
    Article Snippet: The medium was then replaced with a commercial osteoblast differentiation medium (Lonza Group Ltd., Basel, Switzerland), and culture continued for the next 21 days.



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    Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
    Canine Osteoblast Differentiation Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    osteoblast differentiation medium  (Lonza)
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    Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
    Osteoblast Differentiation Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+differentiation+medium/osteoblast+differentiation+medium/pm40362457-488-8-11
    Average 90 stars, based on 1 article reviews
    osteoblast differentiation medium - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    ogmtm osteoblast differentiation medium singlequotstm supplements  (Lonza)
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    Lonza ogmtm osteoblast differentiation medium singlequotstm supplements
    Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
    Ogmtm Osteoblast Differentiation Medium Singlequotstm Supplements, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+differentiation+medium/ogmtm+osteoblast+differentiation+medium+singlequotstm+supplements/pm40265253-60-6-13
    Average 90 stars, based on 1 article reviews
    ogmtm osteoblast differentiation medium singlequotstm supplements - by Bioz Stars, 2026-10
    90/100 stars
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    Image Search Results


    Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

    Journal: Regenerative Therapy

    Article Title: Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins

    doi: 10.1016/j.reth.2025.05.008

    Figure Lengend Snippet: Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

    Article Snippet: The cells were cultured in canine osteoblast differentiation medium (Cell Applications, San Diego, CA, USA) for 28 days at 37 °C and 5 % CO 2 .

    Techniques: Derivative Assay, Staining, Quantitative Proteomics, Standard Deviation, Marker